96-Well Real-Time PCR - qPCR System - 4 or 6 Channels - No ROX Correction - 6 °C/s

The Master Science 96-Well Real-Time PCR is a quantitative PCR system for research, clinical testing, quality and safety analysis and forensic work. It is offered with 4 or 6 fluorescence channels for multiplex assays.

A scanning optical system with time-resolved signal separation and an MPPC detector reduces colour crosstalk and edge effects, so no ROX passive reference correction is needed. Edge temperature compensation helps keep all 96 wells at the same temperature.

Technical Specifications

Specifications of the Master Science 96-Well Real-Time PCR (Q96-5 / Q96-6):

• Brand: Master Science
• Model Number: MSPCR-Q96-5SWISS, MSPCR-Q96-6SWISS
• SKU: MSPCR-Q96-5SWISS, MSPCR-Q96-6SWISS
• Brand: Master Science
• Model Numbers: MSPCR-Q96-5SWISS, MSPCR-Q96-6SWISS
• SKU: MSPCR-Q96-5SWISS, MSPCR-Q96-6SWISS
• Sample Capacity: 96 × 0.1 mL tubes, 8 × 12 strips or one 96-well plate
• Reaction Volume: 10 to 50 µL
• Thermal System: Peltier with edge temperature compensation
• Maximum Ramp Rate: 6.0 °C/s
• Temperature Range: 4 to 100 °C
• Temperature Accuracy: ±0.2 °C
• Uniformity: ±0.2 °C at 60 °C and 95 °C
• Gradient: 30 to 100 °C, span 1 to 36 °C
• Excitation: 5 or 6 high-efficiency single-colour LEDs
• Detector: MPPC, time-resolved signal separation
• Excitation / Detection: 455 to 650 nm / 510 to 715 nm
• Fluorescence Channels: 4 (Q96-5) or 6 (Q96-6)
• Supported Dyes: FAM/SYBR Green, VIC/JOE/HEX/TET, TAMRA/Cy3, ROX/Texas Red, Cy5 and others
• Sensitivity: Single-copy gene
• Resolution: 1.33-fold copy number difference (single-plex)
• Dynamic Range: 10 orders of magnitude
• Package (Q96-5): 960 × 660 × 750 mm, 47 kg gross

Key Benefits

• No ROX needed: the Master Science 96-Well Real-Time PCR separates signals by time-resolved detection.
• Multiplex ready: up to 6 channels.
• Even wells: edge temperature compensation.
• Fine discrimination: detects 1.33-fold copy differences.
• Assay optimisation: 1 to 36 °C gradient.

For a compact 32-well unit, see the Master Science portable real-time PCR.

Product Features

Optics

• Scanning optical system
• Time-resolved signal separation
• MPPC detector

Thermal

• Peltier, 6 °C/s
• Edge temperature compensation
• Gradient 1 to 36 °C span

Assays

• 4 or 6 channels
• Single-copy sensitivity
• 10-log dynamic range

Applications

The Master Science 96-Well Real-Time PCR is used in:

• Scientific research
• Clinical detection and diagnosis
• Food quality and safety testing
• Forensic analysis
• Multiplex PCR assays

Common Laboratory Problems and How This System Helps

How the Master Science 96-Well Real-Time PCR improves qPCR data:

• Crosstalk between dyes: time-resolved separation.
• Edge wells reading differently: edge temperature compensation.
• Extra cost of ROX reference dye: no ROX correction required.
• Limited multiplexing: up to 6 channels.