Filter Fluorescence Spectrophotometer - Emission Scanning Fluorometer - 365 nm Filter - 200-900 nm

The Master Science Filter Fluorescence Spectrophotometer excites the sample through a 365 nm filter and scans the emission from 200 to 900 nm with a Czerny-Turner grating monochromator. It works in two modes, fluorescence and luminous intensity.

Wavelength scanning, time scanning for kinetics and quantitative analysis are all provided. A full emission spectrum can be taken in one second, and a pre-scan finds the emission peak of an unknown sample while skipping scatter and harmonic peaks.

Technical Specifications

Specifications of the Master Science Filter Fluorescence Spectrophotometer:

• Brand: Master Science
• Model Number: MSSP-LF96PSWISS
• SKU: MSSP-LF96PSWISS
• Brand: Master Science
• Model Number: MSSP-LF96PSWISS
• SKU: MSSP-LF96PSWISS
• Emission Monochromator: C-T diffraction grating
• Excitation: 365 nm filter as standard (200-900 nm available as options)
• Emission Wavelength Range: 200 to 900 nm
• Emission Bandwidth: 10 nm
• Sensitivity: S/N ≥150 (P-P), water Raman peak at 365 nm excitation
• Linearity: ≥0.995
• Stability: Better than 1.5% per 10 min
• Excitation Source: 150 W xenon lamp
• Gain: 17 stages
• Response Time: 0.1 to 4 s, 6 stages
• Fluorescence Display Value: 0.00 to 600.00
• Data Transfer: USB 2.0
• Power Supply: 110-240 V AC, 50/60 Hz

Key Benefits

• Quick look at unknowns: the Master Science Filter Fluorescence Spectrophotometer pre-scans to locate the emission peak.
• Wide signal range: 17 gain stages from fast survey to precise scans.
• PC optional: stand-alone intensity and concentration read-out.
• Comparable results: normalised fluorescence values.
• Stable light: long-life xenon lamp and power source.

For scanning excitation as well, see the Master Science compact fluorescence spectrophotometer.

Product Features

Measurement

• Wavelength scanning
• Time scanning for kinetic analysis
• Quantitative analysis
• Automatic skipping of scatter and harmonic peaks

Off-line Mode

• Fluorescence intensity measurement
• Direct concentration reading
• Auto zero
• Automatic background subtraction

On-line Mode

• Qualitative and quantitative software
• Data acquisition and analysis through USB 2.0

Applications

The Master Science Filter Fluorescence Spectrophotometer is used in:

• Clinical analysis of biological specimens
• Pharmaceutical quality control and metabolite research
• Biochemistry: trace substances in biological material
• Food industry: trace constituents
• Environmental and pollution testing
• Trace analysis in organic and inorganic chemistry

Standard Accessories Included

• Main instrument, 1 set
• 365 nm filter, pre-assembled, 1 pc
• Software package, 1 set
• Power cable, 1 pc
• USB cable, 1 pc
• Instruction manual, 1 copy
• Fuses 2 A, 2 pcs; 5 A, 2 pcs
• 10 mm quartz fluorescence cells, 1 pair

Optional Accessories

• Single-hole cell holder
• 200 µL micro centrifuge tube holder
• Micro capillary sample holder
• Semi-automatic sample introduction accessory
• Membrane, powder and jacketed sample accessories
• Other excitation filters, 200-900 nm

Common Problems and How This Instrument Helps

How the Master Science Filter Fluorescence Spectrophotometer supports trace analysis:

• Unknown emission maximum: intelligent pre-scan.
• Scatter peaks mistaken for fluorescence: automatic omission of scatter and harmonics.
• Signals too weak or saturated: 17-stage gain adjustment.
• Results that differ between instruments: normalisation of fluorescence values.