HPLC System - Liquid Chromatograph - DAD 190-800 nm - 1800 lines/mm Grating

The Master Science HPLC Liquid Chromatography System is a modular high-performance liquid chromatograph built for routine quantitative analysis and method development. Pump, autosampler, column oven and detector are separate modules, so a laboratory can start with the configuration it needs today and add detectors or valves later.

It is used for the separation, identification and quantification of compounds in pharmaceutical, food, environmental and chemical samples, with a choice of UV, DAD, fluorescence, refractive index and evaporative light scattering detection.

Technical Specifications

The key specifications of the Master Science HPLC Liquid Chromatography System and its main modules are listed below. Values are taken from the manufacturer's product documentation.

• Brand: Master Science
• Model Number: MSHPLC-W3200SWISS
• SKU: MSHPLC-W3200SWISS
• System Design: Modular HPLC system
• Pump Options: Isocratic pump, binary pump (2 or 4 solvents), quaternary pump
• Pump Technology: High-pressure constant flow pump with secondary suspension; electronic pulsation suppression and solvent compression compensation
• Check Valves: Acetonitrile-resistant check valves
• Gradient Valve: High-precision gradient valve
• Degasser: Integrated, serving the pump and the autosampler wash solvent
• Plunger Cleaning: Built-in online plunger rod cleaning
• Grating: 1800 lines/mm
• Detector Options: UV, DAD, FLD, RID, ELSD
• DAD Wavelength Range: 190 to 800 nm (simultaneous detection)
• UV Detector Light Source: Deuterium lamp and tungsten lamp (dual-lamp design)
• UV Detector Wavelength Range: 188 to 900 nm
• UV Detector Functions: Dual-wavelength detection, full-range wavelength scanning, leak alarm, automatic spectral calibration
• Fluorescence Detector: DC xenon lamp, dual monochromator, touch-screen LCD, dual analog outputs, RS232 or Ethernet data transfer
• Refractive Index Detector: LED light source, temperature-controlled module, leak alarm, digital and analog output, 7-inch touch screen
• ELSD: Low-temperature evaporation, split-flow mode, independent nebulization, evaporation and detection gas control, photomultiplier tube detection, 7-inch touch screen
• Autosampler: High-precision metering pump, polar coordinate design with XYZ movement, multiple injection modes, auxiliary air pump, optional cooling mode, internal lighting
• Column Oven: Bidirectional forced-air heating and cooling
• Column Capacity: 2 × (250 mm column + guard column) or 4 × (150 mm column + guard column)
• Column Oven Options: Switching valves for two-dimensional chromatography and online sample preparation
• Software: Chromatography data system with local or network deployment, database storage, audit trail and multi-level access control

Key Benefits

• Better repeatability between runs: electronic pulsation suppression and solvent compression compensation reduce pressure fluctuation, which helps keep retention times and peak areas consistent.
• Lower running costs: secondary suspension in the high-pressure pump reduces seal wear, and online plunger rod cleaning helps prevent buffer salt deposits that shorten seal life.
• One system for many methods: the Master Science HPLC Liquid Chromatography System accepts five detector types, so the same platform can cover UV-active compounds, fluorescent analytes and compounds with no chromophore.
• Reliable operation with organic solvents: acetonitrile-resistant check valves are designed for the mobile phases most used in reversed-phase work.
• Data integrity support: the chromatography software provides audit trail and multi-level user access, which helps laboratories working under GMP record-keeping requirements.

For other analytical instruments that work alongside an HPLC in the same laboratory, see the Master Science laboratory instrument range.

Product Features

Pump

• Isocratic, binary or quaternary configuration
• High-precision gradient valve for repeatable gradients
• Integrated degasser for pump and autosampler wash solvent
• Acetonitrile-resistant check valves and online plunger rod cleaning

Autosampler

• High-precision metering pump for accurate injection volumes
• Polar coordinate design with XYZ three-dimensional movement
• Several selectable injection methods
• Optional sample cooling and internal lighting for easy observation

Column Oven

• Forced-air heating and cooling for uniform column temperature
• Holds two 250 mm or four 150 mm columns with guard columns
• Accepts switching valves for 2D chromatography and online sample preparation

Detectors

• DAD with 190 to 800 nm range, spectral library, peak purity and 3D views
• UV detector with deuterium and tungsten lamps, 188 to 900 nm
• Fluorescence detector with xenon lamp and dual monochromator
• Refractive index detector with long-life LED source
• ELSD with low-temperature evaporation for thermally unstable compounds

Applications

The Master Science HPLC Liquid Chromatography System suits laboratories that need to separate and quantify compounds in liquid samples, including:

• Pharmaceutical assay, impurity and dissolution testing
• Food analysis, including vitamins, amino acids and aflatoxins (fluorescence detection)
• Environmental testing for polycyclic aromatic hydrocarbons
• Sugar and polymer analysis with refractive index or ELSD detection
• Chemical quality control and research method development

Optional Accessories

• Additional detector modules: UV, DAD, FLD, RID, ELSD
• Column switching valves

Common Laboratory Problems and How This System Helps

The Master Science HPLC Liquid Chromatography System addresses several everyday problems in chromatography laboratories:

• Drifting retention times: pulsation suppression and solvent compression compensation keep flow steady, and the forced-air column oven holds column temperature uniform.
• Frequent pump seal replacement: secondary suspension and online plunger cleaning reduce wear on seals.
• Buying a new instrument for each method: the modular design lets you add a detector instead of a second system.
• Air bubbles and noisy baselines: the integrated degasser treats both the mobile phase and the autosampler wash solvent.
• Audit findings on data records: the software keeps an audit trail and controls user access levels.