Rapid Separation HPLC System - RSLC Liquid Chromatograph - 75 MPa Pump - 190-800 nm DAD

The Master Science Rapid Separation HPLC is a modular rapid separation liquid chromatography (RSLC) system. Its pump withstands up to 75 MPa, so it works with small-particle columns and lets existing methods be transferred without redevelopment.

The autosampler uses a needle-in-flow design in which the whole sample is swept onto the column. Pumps are available as quaternary with 5-channel degassing or binary with a solvent switching valve, and the detector range covers UV, DAD, FLD, ELSD and RID.

Technical Specifications

Specifications of the Master Science Rapid Separation HPLC:

• Brand: Master Science
• Model Number: MSHPLC-W3400SWISS
• SKU: MSHPLC-W3400SWISS
• Brand: Master Science
• Model Number: MSHPLC-W3400SWISS
• SKU: MSHPLC-W3400SWISS
• System Type: Modular rapid separation LC (RSLC)
• Maximum Pump Pressure: 75 MPa
• Pump Options: Quaternary with 5-channel degassing (A, B, C, D, AS); binary with solvent switching valve
• Check Valve: Acetonitrile-resistant, self-restoring
• Autosampler: Needle-in-flow injection with metering pump
• Autosampler Functions: Obstacle protection, empty vial detection, online enrichment, derivatisation, dilution and mixing
• Column Oven: Semiconductor cooling and resistive heating, forced air circulation, eluent preheating
• Column Capacity: Up to 3 columns of 300 mm
• DAD Wavelength Range: 190 to 800 nm, 1024-element photodiode array
• UV Detector Range: 188 to 900 nm with deuterium and optional tungsten lamps
• Fluorescence Detector: Dual monochromator, 4 wavelength channels, xenon lamp
• RID: Two-chamber design, LED light source
• ELSD: Low-temperature evaporation, built-in gas mass flow meter

Key Benefits

• Faster runs on small-particle columns: the Master Science Rapid Separation HPLC delivers up to 75 MPa.
• No sample loss: needle-in-flow injection.
• Low carry-over: optimised needle wash path.
• Stable acetonitrile delivery: dedicated check valve design.
• Reliable integration: intelligent detection of shoulders and small peaks.

For standard-pressure work, see the Master Science quaternary HPLC system.

Product Features

High-Pressure Pump

• Fluid compression compensation with real-time pulsation suppression
• Two-stage floating technology reduces seal wear
• Touch panel switch for stand-alone operation
• Linked purge with the autosampler, no manual purge valve

Autosampler

• Metering pump for precise sampling
• Multiple sample tray formats
• Optional sample cooling
• Optional active condensate drainage

Column Oven

• Heating and cooling in one unit
• Upgradeable to 2D with built-in high-pressure switching valve
• Optional column GLP information recording

Detectors

• DAD with 3D spectra, contour plots and peak purity
• UV with multi-standard wavelength calibration, stop-flow scanning and dual-wavelength detection
• ELSD for thermally unstable and semi-volatile compounds
• RID, FLD and ELSD with touch LCD and analogue/digital outputs

Workstation Software

• Audit trail with records that cannot be edited or deleted
• Automatic backups covering the latest 7 days
• Second-order peak detection with Gaussian, exponential and tangent skimming
• Control of instruments from multiple manufacturers

Applications

The Master Science Rapid Separation HPLC is used for:

• High-throughput liquid chromatography
• Method transfer from conventional HPLC to small-particle columns
• Analysis needing UV, DAD, fluorescence, refractive index or light-scattering detection

Optional Accessories

• UV, DAD, FLD, ELSD and RID detectors
• Autosampler refrigeration unit
• External switching valves
• Chromatography consumables

Common Problems and How This System Helps

How the Master Science Rapid Separation HPLC tackles common LC limitations:

• Pressure limit blocking small-particle columns: 75 MPa pump.
• Carry-over between injections: needle-in-flow design with optimised wash.
• Check valves sticking in acetonitrile: acetonitrile-resistant valve.
• Manual integration of shoulder peaks: intelligent peak detection.